IN VITRO METHOD FOR MODIFICATION OF THE DEPLETION PROFILE OF REGULATORY T CELLS PRESENT IN A TOTAL POPULATION OF SPLENOCYTES IN A SAMPLE, CULTURE AND EXPOSURE OF SAID CELLS TO A MEDIUM WITH ATP AND POLYMYXIN B

摘要

In vitro method to modify the depletion profile of Treg cells present in a total splenocyte population of a biological sample comprising isolating, cultivating and submitting the splenocytes to a medium comprising ATP and polymyxin B, wherein the splenocytes are submitted to concentrations of polymyxin B in the range of 0.1 μg/ml to 1,000 μg/ml, and combined use of polymyxin B and ATP for the preparation of a medicament useful in the treatment of a tumors or cancer in mammals, wherein the cancer is melanoma.