BIOLOGICAL ACTION OF MISSENSE GENOTYPE PERTURBATIONS ON PHENOTYPE

摘要

A method, computer program product, and computer system for determining or classifying a phenotypic effect of a mutation in a protein is disclosed. An action of a protein mutation is calculated as a function of a magnitude of a change at a protein residue and an evolutionary gradient. The evolutionary gradient measures the magnitude of an evolutionary jump associated with the mutation at an amino acid sequence position. The evolutionary gradient also comprises the slope of a tangent to a protein evolutionary trajectory. The phenotypic effect of missense mutations in viral, prokaryotic, and eukaryotic proteins is disclosed.