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METHOD FOR EVALUATING TOXICITY USING EMBRYOS OR LARVAE OF BOMBINA ORIENTALIS

机译:用东印度血吸虫的胚胎或幼虫评估毒性的方法

摘要

The present invention relates to a method for evaluating toxicity using embryos of Bombina orientalis, comprising the steps of: obtaining blastula stage embryos of Bombina orientalis; treating the obtained embryos with inspection material; cultivating the embryos treated with inspection material for H hours at a temperature of T°C so as to develop into larvae; measuring growth rate, survival rate, or teratogenesis rate by comparing with a control group which is not treated with inspection material, wherein the T is 20-23, and the H is 4×T2- 204×T + 2720 - 12 or 4×T2- 204×T + 2720 + 12. The method for evaluating of the present invention uses amphibians indigenous to Korea by replacing exotics amphibian belong to the genus Xenopus, which is widely used in the existing methods for evaluating toxicity, such that reliability of the results in applying the toxicity evaluation results to the domestic natural ecosystem can be remarkaby considered, so that the method can be effectively used as a Korean indigenous toxicity evaluation experiment protocol.;COPYRIGHT KIPO 2014;[Reference numerals] (AA) Breeding;(BB) Blastula embryo (jelly layer removal, embryo selection);(CC) At least five random arrangement;(DD) 20°C ten days;(EE) 22°C seven days;(FF) 23°C six days;(GG) Survival rate and teratogenesis rate;(HH) Measurement analysis;(II) Fixing;(JJ) Chemical material by culture fluid + concentration;(KK) Male red-bellied frog;(LL) Female red-bellied frog
机译:本发明涉及一种利用东方扁桃的胚胎评价毒性的方法,包括以下步骤:获得东方扁桃的囊胚期胚;用检查材料处理获得的胚胎;在T℃下将经检验材料处理过的胚胎培养H小时,以使其发育为幼虫。通过与未用检查材料处理的对照组比较来测量生长率,存活率或致畸率,其中T为20-23,H为4×T2-204×T + 2720-12或4×。 T2-204×T + 2720 + 12。本发明的评价方法使用韩国原产的两栖动物,通过替换属于非洲爪蟾属的两栖动物而被广泛使用,在现有的评价毒性的方法中被广泛使用,以使得其可靠性。可以考虑将毒性评估结果应用于国内自然生态系统的结果,从而可以将该方法有效地用作韩国本土毒性评估实验规程。; COPYRIGHT KIPO 2014; [参考数字](AA)育种;(BB )胚芽胚(胶质层去除,胚选择);(CC)至少五次随机排列;(DD)20°C十天;(EE)22°C七天;(FF)23°C六天;(GG存活率和致畸率;(HH)测量成分分析;(II)固定;(JJ)培养液+浓度的化学物质;(KK)雄性红腹蛙;(LL)雌性红腹蛙

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