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SOLUTION pretreatment immunohistochemical staining and condensed SOLUTION

机译:SOLUTION预处理免疫组织化学染色和浓缩SOLUTION

摘要

1. A solution of pretreatment for immunohistochemical staining of paraffin-containing elution sealing medium from the slide with a tissue sample, drenched in said medium, and for retrieving the antigenicity of said tissue sample, said pretreatment solution may be used three or more times, said a pretreatment solution comprising an agent for extracting the antigens, the nonionic surfactant is polyoxyethylene alkilfenilefir, nonionic surfaces but the active agent of polyoxyethylene sorbitan and a cyclodextrin or a derivative thereof, wherein the remaining amount is not less than 80 wt.% water, wherein the content of said agent for retrieving antigens such that the pH of said solution for the pretreatment is 5.0 to 10, 0, and the content of said cyclodextrin or derivative thereof is from 0.01 to 1.0 wt.%. 2. A solution of pretreatment for immunohistochemical staining of claim 1, wherein the content of said nonionic surfactant is polyoxyethylene alkilfenilefira is from 0.01 to 1.0 wt.%, And the content of said nonionic surfactant is polyoxyethylene sorbitan 0 3 to 3.0 wt.%. 3. A solution of pretreatment for immunohistochemical staining of claim 1, further comprising amphiphilic rastvor.4. A solution of pretreatment for immunohistochemical staining of claim 1, wherein said agent for retrieving antigens is a citrate buffer and / or Tris-bufer.5. A solution of pretreatment for them
机译:1.一种预处理溶液,用于用组织样品从载玻片上对含石蜡的洗脱密封介质进行免疫组织化学染色,浸入所述介质中,并用于回收所述组织样品的抗原性,所述预处理溶液可以使用三次或多次。所述预处理溶液,其包含用于提取抗原的试剂,所述非离子表面活性剂为聚氧乙烯链烷腈,非离子表面但聚氧乙烯山梨糖醇酐的活性剂和环糊精或其衍生物,其中剩余量不少于80重量%的水,其中所述用于抗原回收的试剂的含量使得所述用于预处理的溶液的pH为5.0至10、0,并且所述环糊精或其衍生物的含量为0.01至1.0重量%。 2.权利要求1的用于免疫组织化学染色的预处理溶液,其中所述非离子表面活性剂的含量为0.01至1.0wt。%,并且所述非离子表面活性剂的含量为0 3至3.0wt。%的聚氧乙烯山梨聚糖。 3.根据权利要求1所述的用于免疫组织化学染色的预处理溶液,其进一步包含两亲性拉斯托夫。权利要求1的免疫组织化学染色的预处理溶液,其中所述的抗原回收剂是柠檬酸盐缓冲液和/或Tris-bufer。5。为他们预处理的解决方案

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