METHOD immunoassay particular neuron-specific enolase in a sample of blood plasma for express-diagnostics of early forms of small-cell lung cancer and other cancers WITH TEST SYSTEMS

摘要

1. Test system for the immunoassay determination of neuron-specific enolase in a sample of blood plasma, comprising an immunological plate with a window for introducing a sample and a window for viewing results, which is placed inside the plastic substrate with an adhesive layer for attaching the porous material, in which successive lengths and overlapping membrane arranged for applying the test plasma sample, the membrane for application of gold conjugate antibodies porous membrane and the absorbent membrane, the membrane for applying the conjugate is impregnated with the conjugate of colloidal gold particles with the first monoclonal mouse antibodies specific to neuron-specific enolase, and a porous membrane transversely to the latter and parallel to each other are formed test strip containing adsorbed second mouse monoclonal antibodies specific to neuron-specific enolase, and the control strip containing the adsorbed antibody immunoglobulin myshi.2. Test system according to claim 1 comprises a membrane for applying a sample made of cellulose or fiberglass, or polyester microfibers with a pore size of 20-25 microns and the absorption capacity of not less than 55 mg / see 3. Test system according to claim 1 comprises a membrane for applying a conjugate made from pulp, or fiberglass, or polyester microfibers with a pore size of 20-25 microns and the absorption capacity of not less than 55 mg / see 4. Test system according to claim 1 comprising a porous membrane made of nitrocellulose, or polyethersulfone, or nylon with a layer thickness of 125-155 microns and a liquid flow rate of 75 to 240 sec / 4 sm.5. Test system according to claim 1 comprising absorption