MUTANT CHANNEL RODOPSIN-2

摘要

1. Light-activated ion channel, where the light-activated ion channel contains an amino acid sequence that is at least 70% homologous to the amino acid sequence of positions 1-309 of SEQ ID NO: 1 (CHOP-2), and which contains a substitution at the position corresponding to L132 in SEQ ID NO: 1, where substitution increases the polarity of the channel. 2. The light-activated ion channel of claim 1, wherein the light-activated ion channel comprises preferably the amino acid sequence of positions 1-309 of SEQ ID NO: 1 (CHOP-2), with the exception of the substitution at position L132, where the replacement increases the polarity of the channel. 3. The light activated ion channel according to claim 1, wherein the substitution is selected from L132C, L132S, L132E, L132D and L132T, where, preferably, the replacement is L132C. The light-activated ion channel according to any one of the preceding claims, wherein (a) the photosensitivity is increased by more than 5 times, preferably more than 10 times, more preferably more than 20 times, since 30 times, even more preferably, more than 40 times, since 50 times, and most preferably, more than 60 times or even more than 70 times compared to CHOP-2 WT in hippocampal neurons, and / or (b) calcium conductivity is increased at least two times, preferably at least three times, more preferably at least four times compared to CHOP-2 WT, as determined by imaging Fura-2 on HEK293 cells, and / or (c) the stimulation frequency is increased by at least 1.5 times, more preferably 2 times, or even more preferably 2.5 times compared with CHOP-2 WT, as determined by electrophysiological recordings of a whole cell in hippocampal neurons. 5. The light activated ion channel according to claim 1, where