TARGET GENES FOR FIGHTING PLANTS NEMATODES PARASITING ON PLANTS AND THEIR APPLICATION

摘要

1. An isolated polynucleotide selected from the group consisting of: (a) a fragment of at least 19 adjacent nucleotides of a nucleic acid sequence, any of: SEQ ID NO: 1-9, wherein the contact of a plant-parasitic nematode with a double-stranded ribonucleotide sequence containing at least one chain that is complementary to the specified fragment, inhibits the growth of the specified nematodes; and (b) complement the sequence (a) .2. An isolated polynucleotide according to claim 1, operably linked to a heterologous promoter. An isolated polynucleotide according to claim 1 as part of a plant transformation vector. The double-stranded ribonucleotide sequence produced by expression of the polynucleotide according to claim 1, wherein contacting said ribonucleotide sequence with a plant-parasitic nematode inhibits the growth of said nematode. The double-stranded ribonucleotide sequence according to claim 4, defined as being produced by obtaining a recombinant polynucleotide sequence containing the first, second and third polynucleotide sequence, where the first polynucleotide sequence contains the isolated polynucleotide according to claim 1, where the third polynucleotide sequence is linked to the first polynucleotide sequence via the second polynucleotide sequences, and where is the third polynucleotide sequence This is essentially the reverse complement of the first polynucleotide sequence, so that the first and third polynucleotide sequences hybridize when