Sterilization methods of the bacterial cells with polyethylene glycol-based nonionic surfactant

摘要

In the present invention, by treating a cell or a culture containing the cell with a polyethylene glycol nonionic surfactant, the cell is almost completely killed and the activity of the enzyme expressed in the cell is high. The present invention relates to a method for killing maintained cells and the killed cells. More specifically, the present invention relates to a method for killing bacterial cells so that they can be used for food production by killing the bacterial cells using a polyethylene glycol-based nonionic surfactant that can be used for foods, and It relates to the killed bacterial cell-containing material. In addition, the present invention is used in the tagatose production process by killing Corynebacterium genus cells that produce galactose and / or arabinose isomerase using a polyethylene glycol-based nonionic surfactant. It relates to a possible dead cell content.