Surface plasmon excitation enhanced fluorescence spectroscopy for the sensor chip and the measurement method using the same

摘要

PROBLEM TO BE SOLVED: To provide a sensor chip for SPFS dispensing with previous fractionation of analytes by a column treatment, namely, capable of quickly performing a measurement, preventing loss of the analytes caused by the column treatment, and highly accurately determining the quantity of specific analytes, and to provide a measuring method using the same.;SOLUTION: The sensor chip for a channel type SPFS includes ligands that are fixed on the channel bottom face. The ligand has different binding abilities for each of analytes having two or more types with different sugar chain shapes, and the ligands form a density gradient in the flow direction of the channel.;COPYRIGHT: (C)2013,JPO&INPIT