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Method of producing and purifying soluble recombinant COQ5 protein and soluble recombinant COQ5 protein thereof
Method of producing and purifying soluble recombinant COQ5 protein and soluble recombinant COQ5 protein thereof
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机译:可溶性重组coq5蛋白的生产和纯化方法及其可溶性重组coq5蛋白
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摘要
The present invention relates to a producing and purifying method of soluble recombinant coenzyme Q5 homolog (COQ5) protein, which is expressed in soluble form by Escherichia coli (E. coli), under native conditions. The method is characterized by pre-treating bacterial lysate with low concentration of ionic detergent, such as sodium dodecyl sulfate (SDS), before purification; and the purifying method is performed under native condition without using urea to avoid the problems of requiring lengthy processes to remove urea in purified protein solution or re-aggregation and precipitation of protein after removal of urea.
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