Genetic loci associated with resistance to the soybean cyst nematode and Methods of use

摘要

Claim 1: a method for identifying a first floor or First soybean Germplasm with enhanced resistance to the soybean cyst nematode; The Method comprises: (a) detected in the Genome of soybean plant in the first or the first Genome of soybean Germplasm at least one allele Marker associated with a duplication of a region within the locus rhg1; (b) detected in the Genome ofThe first floor of the first soy or soy Germ plasm a duplication of a region within the locus rhg1; (c) detecting the first Plant soybeans or soybean germplasm in the first an increase in the number of copies in at least one of the SEC with nosIdent: 1, 2, 3, 4, or 5, or a fragment or a variant of These assets; or (d) detected in the Genome of the first floor of the first soy or soy Germ plasm DNA formed a Union at the Breaking Point of a region within the rhg1 duplicated locus.Claim 20: a plant or Plant Cell comprising a heterologous polinucleu00f3tido operably linked to a promoter active in the plant or Plant Cell, where the polinucleu00f3tido heterologous comprises: (a) The Nucleotide sequence as defined in any of the Sec C On nos Ident.1, 2, 3, 4, or 5, or any combination of these; or (b) The Nucleotide sequence that is at least 85% Sequence identity with any of the SEC with nos of ident: 1, 2, 3, 4, or 5, or any combination of these; where the heterologous expression of The polinucleu00f3tido Improvement The Plant resistance to Potato cyst nematode.Claim 23: a method to improve the resistance to cyst nematode in a Plant; The Method comprises introducing into a Plant a heterologous Cell polinucleu00f3tido operably linked to a promoter active in the Plant, wherein the polinucleu00f3tido heterologous underst E: (a) a Nucleotide sequence as defined in any of the SEC with nos Ident.1, 2, 3, 4, or 5, or any combination of these; or (b) a Nucleotide sequence that is at least 85% Sequence identity with any of the SEC with nos, Ident: 1, 2, 3, 4, or 5, or any combination thereof; wherein the expression of polinucleu00f3tido heteru00f3 Logo improves Plant resistance to Potato cyst nematode.Claim 24: The Method according to claim 23, wherein it because (a) is a monocotyledonous plant or Plant Cell; (b) the plant or Plant Cell is corn, Wheat, Rice, Barley, Sugarcane, Sorghum and Rye; (c) the plant or Plant Cell is a dicotil Edu00f3nea; or (d) the plant or Plant Cell is Brassica, Sunflower, cotton and Alfalfa.