Recombinant host cell, methods to manufacture a product and to produce isobutanol, method for the conversion of 2,3 - diidroxiisovalerato in the cetoisovalerato, methods to increase the activity of group of Fe - s in a host cell and the activity of a protein, a method for Indetificar polipet u00ed deos and methods to measure the concetrau00e7u00e3o forms of polypeptides

摘要

The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the FeS cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an FeS cluster biosynthesis pathway in a host cell.