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rf3 gene restorative of maize cytoplasmic male sterility (cms), molecular markers and their use

机译:玉米胞质雄性不育(cms)rf3基因修复,分子标记及其应用

摘要

rf3 gene restorative of maize cytoplasmic male sterility (cms), molecular markers and their use. The present invention relates to a method for selecting a plant comprising a functional restorer gene for maize-type cytoplasmic male sterility comprising the steps of (a) screening a plant population for at least one marker nucleic acid, wherein the marker nucleic acid comprises an allele linked to the functional restorer gene for maize type s cytoplasmic male sterility; (b) detecting the marker nucleic acid; (c) identifying a plant comprising the marker nucleic acid; and (d) selecting the plant comprising the marker nucleic acid, wherein the plant comprising the marker nucleic acid additionally comprises the functional restorer gene for maize type s cytoplasmic male sterility. The present description also provides methods for restoring fertility in a progeny of a type-s male sterile cytoplasmic plant and methods for transferring an rf3 gene to a progeny plant.
机译:玉米胞质雄性不育(cms)rf3基因的修复,分子标记及其用途。本发明涉及一种选择包含用于玉米型细胞质雄性不育的功能性恢复基因的植物的方法,该方法包括以下步骤:(a)筛选植物群体中的至少一种标记核酸,其中所述标记核酸包含等位基因。与功能恢复基因连接的玉米类型的细胞质雄性不育; (b)检测标记核酸; (c)鉴定包含标记核酸的植物; (d)选择包含标志物核酸的植物,其中包含标志物核酸的植物还包含用于玉米s型细胞质雄性不育的功能性恢复基因。本说明书还提供了用于恢复雄性不育细胞质植物的后代中的育性的方法以及将rf3基因转移至后代植物的方法。

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