Set of starters for identification and differentiation of bacteria belonging to the Legionellaceae family and method for application of these starters in the polymerase chain reaction

摘要

the subject of the invention is a set of 21 primers, designed on the basis of 16s rrna gene sequences:, pcs and pmta legionella for identification and differentiation of bacteria of the family legionellaceae and manner of application of these primers in polymerase chain reaction - duplex pcr.a set of primers is characterized by the fact that contain fragments of 16s rrna gene primers, pcs and pmta bacteria with family legionellaceae, of a specified sequence and chain length nukleotydowego have a different degree of konserwatywnou015bci and similar melting temperature, so in u017cyte in various combinations.allow for the detection and differentiation of bacteria of the family legionellaceae response in a given environment.identification and differentiation of bacteria has revealed that polymerase chain reaction in the presence of genomowego dna isolated from environmental samples, involving primers complementary to sequences is to specific regions of the genes used in this j the same reaction mixture in various combinations - systems.