METHOD OF DIAGNOSTIC OF SEVERITY OF LIVER DAMAGE IN PATIENTS WITH DIABETES MELLITUS TYPE 2 (DM2) AND NONALCOHOLIC FATTY LIVER DISEASE (NAZHHP)

摘要

Method of diagnostic of severity of liver damage in patients with diabetes mellitus type 2 (DM2) and nonalcoholic fatty liver disease (NAZHHP) is a laboratory method of diagnosis. It includes selective precipitation of antigen-antibody complexes in 3.75% polyethylene glycol followed by photometric determination of density precipitation, for the reaction are 2 solutions (№ 1-0,1 borate buffer pH = 8,4; № 2-10 g polyethylene glycol M = 6000 was dissolved in 240 ml number 1), a research test tube 0.1 ml of serum of the patient, 0.2 ml and 2.7 number 1 number 2 ml in the control test tube 0.1 ml of patient serum and 2 9 ml number 1, thoroughly shaken and incubated for 1 hour at 2 ° C, at the end of the period of incubation determine the optical density in a spectrophotometer at a wavelength of 450 nm, the result is calculated by the following formula - (O-Kh1000) optical units, where O - index research test tubes; K - rate control tube.