首页> 外国专利> PRODUCTION OF L-XYLULOSE THROUGH COUPLING OF NOVEL NADH OXIDASE DERIVED FROM STREPTOCOCCUS PYOGENES AND L-ARABINITOL OXIDASE

PRODUCTION OF L-XYLULOSE THROUGH COUPLING OF NOVEL NADH OXIDASE DERIVED FROM STREPTOCOCCUS PYOGENES AND L-ARABINITOL OXIDASE

机译:通过链球菌脓疱衍生的新型NADH氧化酶和L-阿拉伯糖醇氧化酶的结合生产L-木糖

摘要

The present invention relates to a novel Streptococcus pyogenes strain producing an NADH oxidase, an NADH oxidase derived from the strain and producing water and a gene thereof, and a method for producing the enzyme, and more specifically, to an NADH oxidase producing water, to a nucleic molecule coding the enzyme, to a vector comprising the nucleic molecule, to a transformant comprising the vector, and to a regeneration of a coenzyme using the NADH oxidase producing water. In addition, it was verified that a high-priced coenzyme is regenerated and repeatedly used through a coupling reaction of an L-arabinitol dehydrogenase producing L-rare saccharides and a novel NADH oxidase producing water, thereby economically producing L-rare saccharides. Further, a purification process can be simplified by using the NADH oxidase producing water to no longer generate byproducts other than water.
机译:本发明涉及产生NADH氧化酶的新型化脓性链球菌菌株,衍生自该菌株并产生水的NADH氧化酶及其基因,以及生产该酶的方法,更具体地说,涉及NADH氧化酶产生水,编码该酶的核酸分子,包含该核酸分子的载体,包含该载体的转化体以及使用产生NADH氧化酶的辅酶的再生。另外,已经证实,通过产生L-阿拉伯糖醇的脱氢酶产生稀有糖的新型酶与新颖的NADH氧化酶的水的偶联反应,可以再生并重复使用高价的辅酶,从而经济地产生L-稀有的糖类。此外,通过使用产生NADH氧化酶的水以不再产生除水以外的副产物,可以简化纯化过程。

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