TAQMAN GENOTYPING ASSAYS FOR THE SCREENING OF COMMON BETA THALASSEMIA MUTATIONS IN MALAYSIAN MALAYS

摘要

The present invention discloses a rapid, sensitive and cost-effective in-house TaqMan genotyping assay for the screening of common β-thalassemia mutations in the Malaysian Malays. The present invention comprises of four sets of forward and reverse primers for DNA amplification of the five common β-thalassemia mutations in Malaysian Malays and five sets of normal and mutant probes. The probes were designed with minor groove binder (MGB) and non-fluorescent quencher (NFQ) at the 3' end, whereas the 5' end contains fluorescence reporter dyes selected from the group of 2-chloro-7-phenyl-l,4-dichloro-6-carboxyfluorescein (VIC), 6- carboxyfluorescein (FAM) or the like.