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METHOD OF CAPTURING CELL FLUID AND ANALYZING COMPONENTS THEREOF WHILE OBSERVING CELL AND SYSTEM FOR CAPTURING AND ANALYZING CELL FLUID
METHOD OF CAPTURING CELL FLUID AND ANALYZING COMPONENTS THEREOF WHILE OBSERVING CELL AND SYSTEM FOR CAPTURING AND ANALYZING CELL FLUID
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机译:观察细胞时捕获细胞流体及其成分的分析方法以及捕获和分析细胞流体的系统
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摘要
SUMMARY OF THE INVENTION;The invention provide the method to enable the molecular detections of cellular fluid of at least a single cell easily and rapidly and clarify the dynamics of life phenomena and molecular mechanisms very rapidly by conducting simultaneous observations of cellular morphological dynamism. At the same time, it provides the method of exploring active molecules. Even in case that the target is a single cell, the cellular fluid is directly sucked by the capillary tip and the fluid is subjected to the analysis directly. By performing this method under the observation of the cell with image-magnifying scopes, such as a microscope, the cellular fluid is sucked and captured by the nanospray ionization capillary tip even if the target is a single cell and the trapped sample is subjected to the mass spectrometer by nano-spraying the sample solution directly after addition of the ionization supporting solvent into the tip, and then the molecular components in the cellular fluid is detected. The detected molecular peaks specific to a certain state are identified by performing differential analysis or t-test between the data groups of cells in different states.
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