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USE OF METE GENE AS AUXOTROPHIC MARKER IN GENETIC CONSTRUCTS
USE OF METE GENE AS AUXOTROPHIC MARKER IN GENETIC CONSTRUCTS
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机译:在基因构建体中使用基因突变体作为营养缺陷标记。
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摘要
A process for the production of a target polypeptide is provided. The process comprises expressing an expression cassette for the target recombinant polypeptide comprised in a vector in a non-mammalian host, wherein the host is auxotrophic for methionine and lacks the ability to express functional chromosomal Vitamin B12 independent homocysteine methyltransferase. The vector comprises an expression cassette for functional Vitamin B12 independent homocysteine methyltransferase and the host is cultured in a growth medium lacking methionine.
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