首页> 外国专利> METHOD FOR ELECTROPHORESING NUCLEIC ACIDS, METHOD FOR CONCENTRATING AND PURIFYING NUCLEIC ACIDS, CARTRIDGE FOR NUCLEIC ACID ELECTROPHORESIS, AND METHOD FOR PRODUCING CARTRIDGE FOR NUCLEIC ACID ELECTROPHORESIS

METHOD FOR ELECTROPHORESING NUCLEIC ACIDS, METHOD FOR CONCENTRATING AND PURIFYING NUCLEIC ACIDS, CARTRIDGE FOR NUCLEIC ACID ELECTROPHORESIS, AND METHOD FOR PRODUCING CARTRIDGE FOR NUCLEIC ACID ELECTROPHORESIS

机译:核酸电泳法,核酸浓缩和纯化方法,核酸电泳法的载体和制备核酸电泳法的方法

摘要

[Object] To provide a method for electrophoresing nucleic acids, which enables highly efficiently concentrating and purifying nucleic acids quickly by simple operation.;[Solving Means] A method of electrophoresis includes electrophoresing a nucleic acid in which an intercalator having an anionic functional group has been inserted. The method includes mixing a sample containing a nucleic acid, a compound having a functional group which undergoes dehydration condensation reaction with carboxyl groups included in substances contained in the sample and a condensing agent of dehydration condensation reaction; and electrophoresing the nucleic acid. The method of concentrating and recovering nucleic acids, by increasing the negative charge of the nucleic acid bound with the intercalator, can increase electrophoretic velocity of nucleic acids. The method of electrophoresing nucleic acids, by subjecting carboxyl groups in the substances in the sample to dehydration condensation reaction, can stably separate nucleic acids and substances by electrophoresis.
机译:目的提供一种电泳核酸的方法,该方法能够通过简单的操作快速高效地浓缩和纯化核酸。[解决手段]电泳方法包括对核酸进行电泳,其中具有阴离子官能团的嵌入剂具有被插入。该方法包括将含有核酸的样品,具有进行脱水缩合反应的官能团的化合物与该样品中所含物质中所含的羧基混合,以及脱水缩合反应的缩合剂进行混合。和电泳核酸。通过增加与嵌入剂结合的核酸的负电荷,浓缩和回收核酸的方法可以提高核酸的电泳速度。通过使样品中的物质中的羧基进行脱水缩合反应的核酸电泳方法可以通过电泳稳定地分离核酸和物质。

著录项

  • 公开/公告号EP2700712A4

    专利类型

  • 公开/公告日2015-01-07

    原文格式PDF

  • 申请/专利权人 SONY CORPORATION;

    申请/专利号EP20120773590

  • 发明设计人 NAKAMURA TOMOHIKO;

    申请日2012-03-28

  • 分类号C12N15/09;C12M1/00;G01N27/447;

  • 国家 EP

  • 入库时间 2022-08-21 15:06:17

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