DEVELOPMENT OF LIGAND SCREENING SYSTEM FOR NEUROTRANSMITTER RECEPTORS

摘要

Provided is a screening method for substances that bind to neurotransmitter receptors, have a group structure represented by formula (II) (in the formula, Rec-Nu represents a group obtained by separating a hydrogen atom from a neurotransmitter receptor (Rec-Nu-H). Nu represents a bivalent group obtained by separating a hydrogen atom from a nucleophile group (Nu-H) possessed by the neurotransmittor receptor. L² represents a bilavent linking group and Fl represents a marker group). A candidate substance, the fluorescence patterns of which vary between when an antagonist is bound and when an agonist is bound, and which is expected to interact with a marked neurotransmitter receptor is made to act on the receptor, the binding mode of the candidate substance and the receptor is detected by variation in the signals emitted by a marker, and screening is carried out based on those results.