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METHOD FOR GUIDING CELL SPREADING IN AUTOMATED CYTOGENETIC ASSAYS
METHOD FOR GUIDING CELL SPREADING IN AUTOMATED CYTOGENETIC ASSAYS
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机译:在自动细胞遗传学检测中指导细胞扩散的方法
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摘要
Provided herein are methods and related systems for controlling droplet spreading on a surface, including droplets in which a biological component is suspended. A biological solution is provided as a droplet to a surface. Interference fringes generated by the droplet on the surface are imaged, wherein the imaging is over a time course during which the droplet spreads on the surface. A droplet parameter is determined from the imaging step and a process parameter controlled to obtain an interference fringe pattern corresponding to a desired droplet parameter. In this manner, well-controlled droplet spreading is achieved, which is important in a range of applications, including assays that rely on good metaphase spreading.
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