Gene Construct for Producing Green Fluorescence Protein-Human Epidermal Growth Factor Fusion Protein and Method of Producing Green Fluorescence Protein-Human Epidermal Growth Factor Fusion Protein Using the Same

摘要

The present invention is green fluorescent protein-gene constructs for the production of human epidermal growth factor fusion protein and green fluorescent protein using the same-way is for the production of human epidermal growth factor fusion protein. Green fluorescent protein according to the invention-human epidermal growth factor fusion protein is expressed as a fusion protein in a stable form within E. coli, and to eliminate the instability of EGF, such as decomposition by a protease in the prior art problems was produced in E. coli during cell The yield was possible in the production of hEGF. In addition, the use of GFP as a recombinant fusion partner protein, separation, and then irradiated with ultraviolet light on a sample of the purification process was able to be easily visually the tracking of a target protein in a sample. In the process according to the invention, as well as a therapeutic agent for chronic intractable skin ulcers such as gastric ulcer, anti-wrinkle and skin improving the reproduction efficiency of the human epidermal growth factor production, which can also be used as a raw material for functional cosmetics for anti-aging and to promote the Hanba simplify the production process, it is useful because it enables the mass production of human epidermal growth factor. ;