A screening method for the optimal surface structure of embryonic stem cell culture using a cell culture plate comprising gradient nanopattern

摘要

The present invention diameters (Diameter) or spacing (Spacing) is constantly changing gradient method for screening a surface structure suitable for an embryonic stem cell culture comprising culturing cells in a cell culture vessel containing the nano-pillar array, the nano-pillar having a constant diameter increases a constant used for the screening method stem-cell function without feeder cells comprising the step of culturing cells in a cell culture vessel containing cell culture plate containing the uniformly arranged at intervals to reduce the nano-pillars of the nano-columns and conditions selected by the screening method ( stemness) while maintaining a method of culturing human embryonic stem cells. Uniformly increasing diameter (Diameter) cell comprises a nano-pillar array of nanopillars by uniformly spacing (Spacing) decreases uniformly with the culture vessel of the invention is to provide a variety of surface types within a culture vessel It is so, may change the surface shape by a single incubation is carried out under the same conditions, monitoring the effects of cell properties at the same time, and it can be selected using the optimum surface shape suitable for the cell culture. Methods of culturing human embryonic stem cells selected by the screening method can provide a coating costly extracellular matrix or which can proliferate stem cells while maintaining cell functions with no need to use a support. ;