The method of evaluating a water quality toxicity of the present invention comprises the steps of placing a water sample in a measuring container; The method comprising the steps of: injecting a frozen rice into a measuring container containing a water sample; Culturing the duckweed in a measuring container; And measuring the rate of change of the growth area or chlorophyll fluorescence of the frozen frozen rice that has been cultivated. Since the method for evaluating the water quality toxicity according to the present invention uses the rate of change of growth area or chlorophyll fluorescence characteristic (especially maximum electron transfer rate) of the frog rice rather than the variation of the population of the frog rice, as the index of the water quality toxicity evaluation, 3 days, and it is shortened by more than two times than conventional water toxicity evaluation time (about 7 days). In addition, it is simpler to measure and more sensitive to the water quality toxicity source because the rate of growth area change or chlorophyll fluorescence is used as an index of water quality toxicity evaluation than colony number variation measurement.
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