The thrombotic dissolving enzyme and its producing method originated in abalone

摘要

The present invention relates to a thrombolytic enzyme using an abalone and a method for producing the thrombolytic enzyme, and more particularly, to a method for preparing a thrombolytic enzyme using an abalone according to an embodiment of the present invention. Adding a predetermined amount of a predetermined concentration of Tris-HCL buffer solution to a predetermined amount of the overturned embryo, and pulverizing the homogenized mixture at a predetermined temperature to obtain a homogenized mixture; A third step of centrifuging the homogenized overturned embryo under a predetermined condition to recover the supernatant; A fourth step of adding a predetermined amount of ammonium sulfate to the recovered supernatant and then centrifuging under a predetermined condition to recover the precipitate; A step of dissolving the recovered precipitate in a predetermined concentration of Tris-HCL buffer and then centrifuging under constant conditions to recover the supernatant from which the impurities have been removed, and concentrating the supernatant under a predetermined condition; And a sixth step of drying the concentrated supernatant.