ASSESSMENT OF THE ACTIVITY OF CELLULAR SIGNAL WAYS USING PROBABILITY MODELING EXPRESSION OF TARGET GENES

摘要

1. The method comprising: removing the activity of one or more cellular signaling pathways in the tissue of a medical subject based on at least the expression level (s) (20) of one or more target genes of the cell signaling pathway (s) measured in the extracted sample tissue of a medical subject, the removal includes: removing the activity of the cell signaling pathway (s) in the tissue of the medical subject by evaluating at least part of the probabilistic model (40-1, ..., 40-7), preferably Bayesian network (40-1, ..., 40-7), representing cell signaling pathway (s) for a number of input data, including at least the expression level (s) (20) of one or more target genes of the cell signaling pathway (s) measured in an extracted tissue sample from a medical subject; rating (46) the level in the tissue of the medical subject of at least one transcription factor (TF) element, wherein at least one TF element controls the transcription of one or more target genes of the cell signaling pathway (s), and the estimate is based at least in part on conditional probabilities linking at least one TF element and expression level (s) (one) of one or more target genes of a cell signaling pathway (s) measured in an extracted tissue sample of a medical subject; deriving activity of a cell signaling pathway (s) based at an estimated level of transcription factor in the tissue sample; determining whether the cellular signaling pathway (s) is functioning incorrectly in the tissue of a medical subject based on the derived activity of the cellular signaling pathway (s) in the tissue of the medical subject;