METHOD OF OBTAINING OF COPOLYMER OF 3-HYDROXYBUTYRATE AND 3-HYDROXYHEXANOATE

摘要

FIELD: biotechnologies.SUBSTANCE: method of obtaining of copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate is offered. The method includes the cultivation of the strain producer Cupriavidus eutrophus VKPM V-10646 in conditions of aeration and stirring in liquid salt water with glucose. At the beginning of the process of fermentation the limiting concentration of sulphur compounds are used , also the substratum-predecessor is added - potassium hexanoate, at the concentration 1 g/l and inhibitor of reactions of beta oxidation cycle - acrylate, at the concentration 0.5-1.0 g/l partially 2-3 times within 1.5-2.5 h. Total duration of the process is 45 hours minimum.EFFECT: high yield of copolymer, decrease of level of crystallinity of copolymer and improvement of elasticity of copolymer.2 cl, 3 dwg, 1 tbl, 2 ex