The invention relates to a time-resolved fluorescence measuring device for high-throughput screening of at least one sample, the device operating with a light source capable of producing a succession of excitation pulses for excitation of the light source. sample so that the sample emits a plurality of fluorescence photons forming a fluorescence emission, the device being characterized in that it comprises: a unit for detecting single photons capable of detecting at least a portion of said photons of fluorescence, the single photon detection unit generating a detection signal for each single photon detected; - a measurement unit for measuring, for each detection signal, the time elapsed between the excitation pulse and the detection signal, called "arrival time", and generating a digital data characteristic of this time of arrival; a unit for real-time analysis of the digital data originating from said measurement unit, comprising means for calculating at least one numerical quantity characteristic of the temporal distribution of the fluorescence emission of the sample.
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