METHOD FOR NONDESTRUCTIVE DETECTION OF miRNA EXPRESSION IN CELL AND DETERMINATION OF CELL TYPE AND STATE

摘要

The present invention provides a method for nondestructive detection of a miRNA expression in a cell and determination of a cell type and state, and specifically provides a method for determining a cell type and state according to a miRNA expression in a cell culture medium. The method comprises: culturing cells of different types, collecting a culture medium of the cells, extracting RNA in the culture medium, performing inverse transcription on the RNA, detecting miRNA in the cell culture medium by using a fluorescent quantitative PCR method, and determining the type and state of the detected cell according to a relationship between miRNA expressions of different cell types and states and the detected miRNA expression. The method of the present invention proves for the first time that the miRNA expression in the culture medium can be detected to determine the type and state of a cell, comprising the pluripotency level of a stem cell and the state of a cell obtained through transdifferentiation, which avoids causing damages to the cell and is especially suitable for experimental and clinical applications where the number of cells is limited.