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Kit and identification method for the identification of pathogenic bacteria of onychomycosis
Kit and identification method for the identification of pathogenic bacteria of onychomycosis
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机译:用于鉴定甲癣病原菌的试剂盒和鉴定方法
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摘要
The present invention aims to provide an identification method and kit for identifying the causative bacteria of onychomycosis which can be used to probe and primer sets for species-specific and identify quickly and accurately the pathogenic bacteria by real-time PCR method and. An identification kit for containing a probe and primer sets, to identify the causative bacteria of onychomycosis using real-time PCR, primer set, the base sequence of SEQ ID NO: 2 and a primer having a nucleotide sequence of SEQ ID NO: 1 primer set comprising a primer with, and are at least one selected from the group consisting of primer set consisting of primers having the nucleotide sequence of SEQ ID NO: 4 and a primer having a nucleotide sequence of SEQ ID NO: 3, the probe , a probe having a base sequence of SEQ ID NO: 5, and is at least one selected from the group consisting of probes having the nucleotide sequence of SEQ ID NO: 6, identifying kit bacterium causes onychomycosis.
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机译:本发明的目的是提供一种用于鉴定甲癣病原菌的鉴定方法和试剂盒,该鉴定方法和试剂盒可用于探针和引物对物种特异性的鉴定,并通过实时PCR方法快速,准确地鉴定病原菌。一种鉴定试剂盒,其包含探针和引物组,以使用实时PCR鉴定甲虫病的致病菌,引物组,SEQ ID NO:2的碱基序列和具有SEQ ID NO:1引物的核苷酸序列的引物所述探针组包括:引物组,所述引物组具有并且选自由具有SEQ ID NO:4的核苷酸序列的引物和具有SEQ ID NO:3的核苷酸序列的引物组成的组中的至少一种。具有SEQ ID NO:5的碱基序列的探针,并且是选自具有SEQ ID NO:6的核苷酸序列的探针的至少一种,其鉴定试剂盒细菌引起甲癣。
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