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Kit and identification method for the identification of pathogenic bacteria of onychomycosis

机译:用于鉴定甲癣病原菌的试剂盒和鉴定方法

摘要

The present invention aims to provide an identification method and kit for identifying the causative bacteria of onychomycosis which can be used to probe and primer sets for species-specific and identify quickly and accurately the pathogenic bacteria by real-time PCR method and. An identification kit for containing a probe and primer sets, to identify the causative bacteria of onychomycosis using real-time PCR, primer set, the base sequence of SEQ ID NO: 2 and a primer having a nucleotide sequence of SEQ ID NO: 1 primer set comprising a primer with, and are at least one selected from the group consisting of primer set consisting of primers having the nucleotide sequence of SEQ ID NO: 4 and a primer having a nucleotide sequence of SEQ ID NO: 3, the probe , a probe having a base sequence of SEQ ID NO: 5, and is at least one selected from the group consisting of probes having the nucleotide sequence of SEQ ID NO: 6, identifying kit bacterium causes onychomycosis.
机译:本发明的目的是提供一种用于鉴定甲癣病原菌的鉴定方法和试剂盒,该鉴定方法和试剂盒可用于探针和引物对物种特异性的鉴定,并通过实时PCR方法快速,准确地鉴定病原菌。一种鉴定试剂盒,其包含探针和引物组,以使用实时PCR鉴定甲虫病的致病菌,引物组,SEQ ID NO:2的碱基序列和具有SEQ ID NO:1引物的核苷酸序列的引物所述探针组包括:引物组,所述引物组具有并且选自由具有SEQ ID NO:4的核苷酸序列的引物和具有SEQ ID NO:3的核苷酸序列的引物组成的组中的至少一种。具有SEQ ID NO:5的碱基序列的探针,并且是选自具有SEQ ID NO:6的核苷酸序列的探针的至少一种,其鉴定试剂盒细菌引起甲癣。

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