Separation system and separation method for multidimensional liquid chromatography for protein separation

摘要

PROBLEM TO BE SOLVED: To provide a separation system and separation method for multidimensional liquid chromatography for protein separation. A multi-dimensional liquid chromatography separation system for protein separation includes a mobile phase reservoir (2), an infusion device No. 1 (3, 1-2) and an infusion device No. 2 (9, 1-1), Sample supply device No. 1 (4-1, 4-2) and sample supply device No. 2 (12), separation device (7), storage device (10), at least two drain devices (7-1, 7- 2,7-3) and channel switching device (11). In the case of performing liquid chromatography separation by dimension, the storage device stores the target middle distillate to be subjected to the next liquid chromatography separation in the storage device, and the liquid chromatography in the second dimension or more dimensions. In the case of separation, the mobile phase transports the infusion device No. 1 and the infusion device No. 2 by mixing all middle distillate from the accumulator with the mobile phase by the sample supply mixer of the sample supply device No. 2. The concentration of the eluent in the feed sample mixture to be lower than the critical transfer eluent concentration of all target proteins retained in the dimension separation at the target middle distillate. To do. [Selection] Figure 2