A novel ninhydrin reagent for the analysis of nitrogen-containing compounds

摘要

A ninhydrin reagent for use in a method for analyzing nitrogen-containing compounds, in particular for visualizing nitrogen-containing compounds by a color-forming reaction, comprising reducing ninhydrin at a first temperature with ninhydrin, an aqueous buffer, and A ninhydrin reagent comprising a temperature-dependent reducing agent, wherein the ninhydrin has an activity to reduce ninhydrin to hydrin dantine at a second temperature, the second temperature being higher than the first temperature. Is done. This reagent is particularly useful in amino acid analysis.