Method for Improving the Specific Effector Functions of Single-Chain-Antigen-Recognizing Genetic Constructs (scARC) Through Murinization Thereof

摘要

The present invention relates to a method for producing a cell line expressing a stabilized functional single chain-antigen-recognizing genetic construct (scARC), comprising a genetic construct of the human scARC to be expressed, comprising the domains huV_1/2-Li-huV_2/1-C_4/3 and a genetic construct comprising the corresponding hetero-/(homo-)dimeric domain C_3/4, containing xenogenic, in its special case murine amino acid exchanges in the domains C_4/3 and C_3/4, wherein co-expression of the genetic constructs of the scARC-fragments occurs through the cell. Preferably, the scARCs are single chain-TCRs (scTCRs) or antibody-scFv-fragments, which further preferably recognize tumor associated peptide antigens (TAA). The present invention further relates to a gp100-protein-specific T-cell response mediated α/β T-cell receptor rationally mutated by means of the method of the present invention and its uses.