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Method for selective derivatization of oligohistidine sequence of recombinant proteins
Method for selective derivatization of oligohistidine sequence of recombinant proteins
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机译:重组蛋白寡组氨酸序列的选择性衍生方法
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摘要
Methods and compositions for the selective derivatization of a oligohistidine-tagged recombinant protein. A modifying compound comprised of an imidazole reactive group, a linker, and a ligating group is contacted with the recombinant protein, and a cooperative bond forms between the ligating group and the oligohistidine tag in the presence of a metal cation, and a covalent bond forms between the imidazole reactive group and an imidazole ring of the oligohistidine tag followed by the concomitant separation of the imidazole reactive group from the linker. Addition of a metal chelator results in the dissociation of the ligating group and the oligohistidine tag.
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