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Cellulase enzyme-based method for the production of alcohol and glucose from pretreated lignocellulosic feedstock
Cellulase enzyme-based method for the production of alcohol and glucose from pretreated lignocellulosic feedstock
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机译:基于纤维素酶的从预处理木质纤维素原料生产乙醇和葡萄糖的方法
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摘要
A process for the production of glucose from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the hydrolyzate slurry in a fermentation reaction to produce a fermentation broth comprising alcohol. A process stream is obtained comprising unhydrolyzed cellulose, which is then subjected to a denaturing step, preferably comprising exposing the unhydrolyzed cellulose to elevated temperatures, thereby producing a heat-treated stream comprising the unhydrolyzed cellulose. The heat-treated stream comprising unhydrolyzed cellulose is then further hydrolyzed with cellulase enzymes to hydrolyze the cellulose to glucose.
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