PURIFICATION AND IMMOBILIZATION OF RING CLEAVING ENZYME FOR DETOXIFICATION OF AROMATIC RING CONTAINIG PESTICIDES

摘要

The invention is for removing PNP in places where methyl-parathion is used. The enzyme has been isolated, which metabolizes, PNP to HQ. HQ is ring cleaved and finally mineralized to CO2. The enzyme is probably ring cleaving enzyme that cleaves ring of HQ. The enzyme is immobilized on cotton muslin cloth, the cloth filter can be used for removal of both PNP and HQ. PNP-4-hydroxylase and hydroquinone dioxygenase are immobilized for this purpose. The invention describes the purification of a dioxygenase. The invention is useful for detoxification of aromatic ring containing pesticides.