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METHODS FOR DIRECT PCR USING A POLYMERIC MATERIAL AS SAMPLE CARRIER.
METHODS FOR DIRECT PCR USING A POLYMERIC MATERIAL AS SAMPLE CARRIER.
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机译:使用聚合物材料作为样品载体的直接PCR方法。
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摘要
A method for amplifying nucleic acid from a higher eukaryotic, such as mammalian or plant, nucleic acid source, the method comprising (a) contacting a sampling device with the source of higher eukaryotic, such as mammalian or plant, nucleic acid such that following said contacting, higher eukaryotic such as mammalian or plant nucleic acid-containing material is adhered to at least part of the sampling device, wherein the sampling device, or part thereof to which the nucleic acid-containing material is adhered, is made of a suitable polymeric material; (b) introducing the sampling device or part thereof to which the nucleic acid- containing material is adhered into a reaction vessel which contains a reaction mixture for carrying out a nucleic acid amplification reaction, without any prior treatment of the nucleic acid-containing material; and (c) performing a nucleic acid amplification reaction.
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