Method for diagnosis and prediction of cerebellar ataxia

摘要

in vitro method for diagnosing and / or predicting hereditary cerebellar ataxia in a dog, comprising determining the presence or absence of a genetic variation homozygous in gene sequence arylsulfatase G in a biological sample from said dog, compared with the gene sequence arylsulfatase G of a healthy dog ​​no carrier, wherein the presence of such genetic variation homozygous indicates said dog is or will be affected by hereditary cerebellar ataxia, said dog a selected race in the group consisting of American Staffordshire terrier, American Pit Bull Terrier and Pit Bull type; wherein the cDNA sequence of the gene Arylsulfatase G of a healthy dog ​​no carrier is as shown in SEQ ID NO: 1 or the genomic gene sequence arylsulfatase G of a healthy dog ​​no carrier is as shown in SEQ ID NO: 2; and wherein said genetic variation is a polymorphism of a single nucleotide (SNP) that is an allele of adenosine at nucleotide position 296 of the cDNA sequence of the gene Arylsulfatase G of SEQ ID NO: 1 or an allele adenosine at nucleotide position 22139 of the genomic sequence of gene arylsulfatase G of SEQ ID NO: 2.