SEX SORTED SPERM DEMONSTRATING A DOSE RESPONSE AND METHODS OF PRODUCING SEX SORTED SPERM DEMONSTRATING A DOSE RESPONSE

摘要

Embodiments of the claimed invention relate to sex sorted sperm having animproveddose response which may be produced by the steps of extending a sperm samplewith abuffering holding media and adjusting the concentration of the extended spermsample to atarget concentration range. The extended sperm may then be stained with a DNAselectivedye and sex sorted with a flow cytometer into a catch fluid. During theextending and sortingof the sperm sample, the pH may be maintained at a target pH. Additionally, atleast one ofthe buffering holding media, the DNA selective dye and the catch fluid may besupplementedwith at least one antioxidant.