The present invention provides a chromatographic separation process for recovering polyunsaturated fatty acid (PUFA) products from a feed mixture, said process comprising the steps of, as eluant, a simulated or actual process with multiple linked chromatography columns containing an aqueous organic solvent Introducing the feed mixture into a mobile bed chromatographic apparatus wherein the apparatus has a plurality of zones including at least a first zone and a second zone, (A) an extraction residue stream containing said PUFA product along with a larger polar component is collected from a column in said first zone, and wherein said second zone / RTI and / or RTI ID = 0.0 (b) An extract stream containing the product is collected from the column in the second zone and introduced into a column not adjacent to the first zone and the PUFA product is separated from the other components of the feed mixture in each zone, The aqueous organic solvent is other than an aqueous alcohol.
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