METHODS FOR PRODUCING AND APPLYING POPULATIONS OF MULTIPOTENT AND PLURIPOTENT CELLS RESISTANT TO DISEASES AND ABLE TO DIFFERENTIATE

摘要

1. A method for producing differentiating cells by cell reprogramming, comprising the steps of: - selecting non-pluripotent somatic cells; - overexpressing or introducing nucleic acid (s) or protein (s) corresponding to one or more transcription factors, usually present in desired cells. 2. The method of claim 1, wherein said cells and / or their progeny overexpress the transcription factor of proteins or nucleic acids selected from the group consisting of: a. Mash1 (Ascl1) or part thereof; b. MyoD, myogenin, Myf5, Myf6, Mef2, myocardin, Ifrd1, muscle cell transcription factor and / or sequence encoding muscle cell specific bHLH or part thereof; c. Foxa2, Sox17, HLXB9 and / or Pdx1 or parts thereof; d. hepatic nuclear factor (HNF) -1, HNF-3, HNF-4, FTNF-6 and / or CREB-binding protein or parts thereof; e. Runx1 / AML1 and / or NOV (CCN3) or parts thereof; f. Sox9, CREB-binding protein, Gata6 and / or Runx2 or parts thereof; g. OLIG1, OLIG2 and / or Zfp488 or parts thereof; h. MyoD, myogenin, myocardin, Myf5, Myf6, Mef2A, Gata4, Gata5 and Gata6 or parts thereof; i. Gata4, Gata5 and / or Gata6 or parts thereof; j. myocardin or part thereof; k. myogenin and MyoD or parts thereof; l. Runx2 or part thereof; m. Mash1 (Ascl1), Ngn2, Nurr1, Lmx1b and / or Ptx-3 or parts thereof; n. Mash1 (Ascl1), Phox2b, Lmx1b, Nkx2.2, Pet1, Gata2, Gata3 and / or Pet1 or parts thereof; o. Mash1 (Ascl1), Phox2a and / or REST4 or parts thereof; p. Mash1 (Ascl1), PITX2, Dlx2, Dlx5, artificial oligonucleotides targeted to HES1, Phox2a and / or REST4 or parts thereof; q. Mash1 (Ascl1), Phox2a, Phox2b, dHand, Gata2 and / or Gata3 or parts thereof; r. Mash1 (Ascl1), Nurr1, REN, neurogenin 1, neurogenin 2, neurogenin 3, Mash1, Phox2b, Phox2a, dHand, Shh, FGF8, Lmx1b, Nkx2.2, Pet1, Lbx1, Rnx, Gata3 and / or antisense RNA parts thereof; s. PRR-Numb or part thereof; or t. Numblike or part thereof. 3. A method according to claim 1, characterized in that said cells and / or their progeny are incubated in a differentiating medium. A method according to claim 1, characterized in that said cells and / or their progeny are incubated in a differentiating medium comprising one or more agents selected from the group consisting of. retinoic acid, neurotrophin 3 (NT3), factor ro