DEVICE FOR rapid determination of the concentration of reactive oxygen in aqueous solutions

摘要

The technical solution relates to the field of Physical and Colloid Chemistry, aqueous solutions, biology, pharmacology, medicine, food industry, namely to the devices for the study of reactive oxygen species (ROS) in aqueous solutions, including those containing metal nanoparticles and other nanomaterials, in the presence of substances with antioxidant activity. This device allows the pH-dependent chemiluminescent reaction is used to detect ROS at given pH values, which increases the reproducibility of the results obtained and can greatly increase the sensitivity of current methods. reactive oxygen species formation is determined by the intensity of the luminescence occurring after adding to the sample solution luminol, which is a reagent for the chemiluminescent detection of reactive oxygen species and a solution of sodium hydroxide or potassium enhancing the luminescence intensity of luminol oxidation reaction, which considerably increases the sensitivity of detection of ROS. Quantitative determination of the oxygen concentration of the active form held by means of additives, by further adding to the reaction mixture a certain amount of its reference solution, measurement of luminescence intensity of the solution with the addition of the standard solution and the corresponding calculation. This device can be used for rapid determination of antioxidant activity of water-soluble substances, and assessment of allergenicity of nanomaterials toxicity as well as for studies of the formation of reactive oxygen species in aqueous solutions. Yl 3. 3 ist. inf.