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METHODS FOR MAKING A TISSUE ENGINEERED MUSCLE REPAIR (TEMR) CONSTRUCT IN VITRO FOR IMPLANTATION IN VIVO
METHODS FOR MAKING A TISSUE ENGINEERED MUSCLE REPAIR (TEMR) CONSTRUCT IN VITRO FOR IMPLANTATION IN VIVO
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机译:体外制作组织工程化的肌肉修复(TEMR)结构的方法
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摘要
Provided herein are methods of culturing organized skeletal muscle tissue from precursor muscle cells by cyclically stretching and relaxing said muscle cells on a support in vitro for a time sufficient to produce said organized skeletal muscle tissue, including reseeding said organized skeletal muscle tissue by contacting additional precursor muscle cells to said organized skeletal muscle tissue on said solid support, and then repeating said step of cyclically stretching and relaxing said muscle cells in said support in vitro for time sufficient to enhance the density (i.e., increased number of nuclei and/or number of multinucleated cells) of said organized skeletal muscle tissue on said support.
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