METHOD FOR PRODUCING PANCREATIC BLAST CELLS AND PANCREATIC DISEASE TREATMENT AGENT CONTAINING PANCREATIC BLAST CELLS

摘要

Provided is a method for generating pancreatic bud cells, having the step of culturing PDX1⁺/NKX6.1^- cells in a medium containing KGF, EGF and a BMP inhibitor. The culturing step may be performed in suspension cultures or in adherent cultures. When the cells are cultured in adherent cultures, the cells may be cultured in a medium further containing a ROCK inhibitor or a nonmuscle myosin II inhibitor.