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METHOD OF PACKING CHROMATOGRAPHIC COLUMNS, PACKED CHROMATOGRAPHIC COLUMNS FOR USE AT HIGH PRESSURES AND USES THEREOF
METHOD OF PACKING CHROMATOGRAPHIC COLUMNS, PACKED CHROMATOGRAPHIC COLUMNS FOR USE AT HIGH PRESSURES AND USES THEREOF
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机译:包装色谱柱的方法,袋装色谱柱在高压下使用及其用途
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摘要
A method of packing a chromatography column, including: dispersing chromatographic media particles in a slurry solution to form a slurry, filling a chromatography column with the chromatographic media particles by introducing the slurry to the column and applying a pressure to pack the chromatographic media particles in the chromatography column. The slurry solution is preferably aqueous based slurry solution. The pressure is preferably applied and held substantially constant for a first period at a first pressure and is applied and held substantially constant for a second period, following the first period, at a second pressure higher than the first pressure.
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