The present invention relates to a biomolecule for labeling rare earth nanomaterials, a labeling method thereof, and a rare earth material dissolution / enhanced time-resolved fluorescence immunoassay method. It has the advantages of specific surface area, excellent modification and low cost, and each nanocrystal contains thousands of lanthanoid ions, so the labeling ratio of rare earth ions is greatly improved. Since it is less affected by exogenous rare earth ions and is not affected by anticoagulants, the applicability is excellent. After forming an immune complex with rare earth nanomaterial as a labeling substance, the rare earth nanomaterial is dissolved in the rare earth ion by adding the enhancement liquid, and a chelate and a new signal molecule in the enhancement liquid are formed. Thus, since energy transfer between molecules is performed, the fluorescence is enhanced nearly a million times, and the fluorescence signal is measured using time resolution, and the measurement sensitivity is greatly improved.
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