METHOD OF ISOLATING PROBIOTICS OF HUMAN INTESTINE

摘要

A method of isolating probiotics of human intestine, including preparing bases for culturing liquid and solid mucoproteins; and filtering, which includes collecting human feces, planting the human feces in the base for culturing liquid mucoprotein, culturing the human feces to obtain microbial liquid, using the microbial liquid as template, pouring the microbial liquid into tubes, subjecting the tubes to a PCR test, selecting the tubes tested positive in the PCR test, pouring the microbial liquid in the positive tubes into the base for culturing solid mucoprotein, culturing the microbial liquid to obtain first colonies, planting the colonies in a chocolate tablet, culturing the first colonies to obtain second colonies, planting the second colonies in a Columbia blood tablet, purifying the second colonies to obtain third colonies, using 16sRNA common primers to identify the third colonies in the PCR, and isolating probiotics.