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TWO-COLOR FLUORESCENCE LOCALIZATION SUPER-RESOLUTION BIOLOGICAL MICROSCOPY METHOD AND SYSTEM
TWO-COLOR FLUORESCENCE LOCALIZATION SUPER-RESOLUTION BIOLOGICAL MICROSCOPY METHOD AND SYSTEM
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机译:两色荧光定位超分辨生物显微镜的方法和系统
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摘要
A two-color fluorescence localized super-resolution biological microscopy method and system are disclosed. The method includes: performing two-color fluorescence labeling on a biological sample by using Alexa647 and Alexa750 fluorescent molecules or Cy5 and Cy7 fluorescent molecules, and soaking the biological sample in an imaging buffer solution; illuminating the biological sample by laser to generate a flashing fluorescent signal of a first channel and a flashing fluorescent signal of a second channel respectively; constructing a super-resolution image of a first biological structure and a super-resolution image of a second biological structure respectively; and aligning the super-resolution image of the first biological structure and the super-resolution image of the second biological structure so as to construct a super-resolution image of a third biological structure. Thereby, the present method and system do not generate channel crosstalk and can significantly reduce background noise, so that the imaging quality is improved.
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