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METHOD OF DETECTING A MICROORGANISM IN A SAMPLE BY A FLUORESCENCE BASED DETECTION METHOD
METHOD OF DETECTING A MICROORGANISM IN A SAMPLE BY A FLUORESCENCE BASED DETECTION METHOD
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机译:荧光检测法检测样品中的微生物的方法
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摘要
The present invention relates to a method of detecting a microorganism, in particular Staphylococcus aureus, in a sample, comprising the steps of a) incubating the sample with a slow off-rate modified aptamer (SOMAmer) comprising a fluorescent label, b) optionally washing the sample, c) analyzing the sample by a fluorescence-based detection method. The invention further relates to a slow off-rate modified aptamer comprising a fluorescent label, wherein the SOMAmer comprises a nucleotide sequence specific for Staphylococcus aureus, its use for detecting Staphylococcus aureus cells in a sample, and to a microarray or biosensor and a kit comprising at least one of such SOMAmers comprising a fluorescent label.
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